| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph
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| Autores: |
Raffoul,Tássia
Swiech,Kamilla
Arantes,Mabel Karina
Sousa,Álvaro Paiva Braga de
Mendonça,Ronaldo Zucatelli
Pereira,Carlos Augusto
Suazo,Cláudio Alberto Torres
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| Data: |
2005-06-01
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| Ano: |
2005
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| Palavras-chave: |
Animal cell
CHO-K1
Microcarrier
Growth
Attachment
Hemolymph
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| Resumo: |
The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132005000400011
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.48 n.spe 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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